Descriptions
5’... G↓AATTC...3’
3’... CTTAA↑G…5’
J-GENEFAST EcoRI restriction enzyme recognizes G^AATTC site and cuts best at 37°C in 5–15 minutes using CutJZTM Buffer or Easy-loadTM CutJZTM Buffer
ECORI is inactivated by incubation at 65°C for 20 min.